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Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

Quanlin DAI, Baifei HUANG, Zhongyi YANG, Jiangang YUAN, Junzhi YANG

《环境科学与工程前沿（英文）》 2010年 第4卷 第4期   页码 449-458 doi: 10.1007/s11783-010-0250-x

摘要： A maize variety, Huatian-1, had an unusually low translocation rate of cadmium (Cd) (59.6 mg·kg in the roots and 0.093 mg·kg in the grain) compared to 24 other varieties while being grown in soils with 16.50 mg·kg Cd. This indicates that this particular species may have special mechanisms that affect the absorption and translocation pattern of Cd. In this paper, the technique of suppression subtractive hybridization (SSH) was used to isolate and identify Cd-induced genes from Huatian-1 hydroponically ?exposed? to? 0.1 mM ?CdCl ?for? 1 h,? 12 h, 24 h, and 48 h. We found a total of 15 differentially expressed genes in the four groups; 2, 3, 4, and 6 genes were from the groups of 1 h, 12 h, 24 h, and 48 h treatment, respectively. Phospholipase mRNA, adenosine triphosphate (ATP) phosphoribosyl transferase 2, and were turned on in the maize in response to Cd stress, and it might provide new clues to explain the mechanism of maize tolerance to Cd.

关键词： Zea mays L.     cadmium (Cd)     suppression subtractive hybridization    

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深碳观测计划——对地球内部碳的十年探索

Schiffries, Andrea Johnson Mangum, Jennifer L. Mays, Michelle Hoon-Starr, Robert M. Hazen

《工程（英文）》 2019年 第5卷 第3期   页码 372-378 doi: 10.1016/j.eng.2019.03.004

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In memory of Perry L. McCarty, seminal founder of Environmental Biotechnology

《环境科学与工程前沿（英文）》 2023年 第17卷 第10期 doi: 10.1007/s11783-023-1730-0

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Comments on the special issue on forestry of

John L. INNES

《农业科学与工程前沿（英文）》 2017年 第4卷 第4期   页码 502-503 doi: 10.15302/J-FASE-2017189

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Shear design of high strength concrete prestressed girders

Emad L. LABIB,Hemant B. DHONDE,Thomas T. C. HSU,Y. L. MO

《结构与土木工程前沿（英文）》 2014年 第8卷 第4期   页码 373-387 doi: 10.1007/s11709-014-0087-7

摘要： Normal strength prestressed concrete I-girders are commonly used as the primary superstructure components in highway bridges. However, shear design guidelines for high strength PC girders are not available in the current structural codes. Recently, ten 7.62 m (25 feet) long girders made with high strength concrete were designed, cast, and tested at the University of Houston (UH) to study the ultimate shear strength and the shear concrete contribution ( ) as a function of concrete strength ( ). A simple semi-empirical set of equations was developed based on the test results to predict the ultimate shear strength of prestressed concrete I-girders. The UH-developed set of equations is a function of concrete strength ( ), web area ( ), shear span to effective depth ratio ( / ), and percentage of transverse steel ( ). The proposed UH-Method was found to accurately predict the ultimate shear strength of PC girders with concrete strength up to 117 MPa (17000 psi) ensuring satisfactory ductility. The UH-Method was found to be not as overly conservative as the ACI-318 (2011) code provisions, and also not to overestimate the ultimate shear strength of high strength PC girders as the AASHTO LRFD (2010) code provisions. Moreover, the proposed UH-Method was found fairly accurate and not exceedingly conservative in predicting the concrete contribution to shear for concrete strength up to 117 MPa (17000 psi).

关键词： shear design     high strength concrete     prestressed girders     full-scale tests    

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Deeply missing my career idol and role model ― Professor Perry L. McCarty

《环境科学与工程前沿（英文）》 2023年 第17卷 第9期 doi: 10.1007/s11783-023-1717-x

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Urban watershed management under rapid urbanization

Pengfei DU, Haifeng JIA, Shaw L. YU

《环境科学与工程前沿（英文）》 2012年 第6卷 第5期   页码 595-595 doi: 10.1007/s11783-012-0452-5

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地下工程的发展提升现代生活质量

Raymond L. Sterling

《工程（英文）》 2017年 第3卷 第6期   页码 780-781 doi: 10.1016/j.eng.2017.12.006

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A Standard for the Commissioning Process

William L. Gillis,Elizabeth A. Cudney

《工程管理前沿（英文）》 2015年 第2卷 第1期   页码 39-51 doi: 10.15302/J-FEM-2015006

摘要： The demand for commissioning services for new-building construction projects is experiencing rapid growth. Commissioning (Cx) is touted as being a quality-focused process for ensuring the owner’s project requirements (OPR) are met by design, final construction, and the operations of a building. To an owner this is just what is needed to receive a perfect building at occupancy. However, as many owners have realized, the Cx process does not guarantee the completed building will be what was expected. It should be pointed out that this is typically not caused by the Cx process, but the Cx process should or could have identified, in the early phases of the project, many of the issues that made it through to the completed building. There are a number of reasons why the Cx services received may not be optimal. Often it is poor communication and the transfer of knowledge between project teams. Cx should and can facilitate both communication and the transfer of knowledge from phase to phase. An adaptation of the quality function deployment (QFD) four-phase model can accomplish this by filling the gaps among the major Cx activities and provide a standard approach to the process. The four-phase model effectively links each of the Cx activities to each other and back to the OPR, providing a method for improved communication and knowledge transfer. This paper investigates some potential reasons for inconsistent Cx services, presents an argument for the need of a Cx standard, and proposes a potential standard.

关键词： construction     engineering management     quality     commissioning    

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The Decarbonization of Construction—How Can Alkali-Activated Materials Contribute?

John L. Provis,Susan A. Bernal,Zuhua Zhang,

《工程（英文）》 doi: 10.1016/j.eng.2023.09.014

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Quality Function Deployment Implementation in Construction: A Systematic Literature Review

Cudney,William L. Gillis

《工程管理前沿（英文）》 2016年 第3卷 第3期   页码 224-230 doi: 10.15302/J-FEM-2016036

摘要： The construction of new buildings represents a significant investment. The goal of new building construction is to maximize value and minimize cost while staying on time and within budget. Translating customer requirements into engineering terms for new construction design is vital for a construction project to be successful. Quality function deployment has been successfully used in product development to capture the voice of the customer and translate it into engineering characteristics. Quality function deployment then carries these parameters into production and service to ensure the voice of the customer is being met with the final product. The house of quality, a tool within quality function deployment, can provide a means for comparison of owner’s project requirements and the proposed design, along with identifying how the design decisions impact meeting customer requirements and green building requirements. Quality function deployment can effectively link the project phases through design and construction and into operations and maintenance to ensure the owner’s project requirements are met with the final building. This research identifies and categorizes studies of quality function deployment applications in construction. The research method used is a systematic literature review from databases related to quality function deployment in the construction industry published in the periodicals through 2016. The principal findings of implementations, practices, and integrated approaches are then summarized. This article intends to propel further research of quality function deployment in the construction sector.

关键词： construction     engineering management     quality function deployment     systematic review    

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Effects of iron oxide nanoparticles on phenotype and metabolite changes in hemp clones ( L.)

《环境科学与工程前沿（英文）》 2022年 第16卷 第10期 doi: 10.1007/s11783-022-1569-9

摘要：

● Fe3O4 NPs increased the biomass and chlorophyll content of hemp clones.

关键词： Fe3O4 nanoparticle     Hemp     Growth enhancement     THC     Metabolite    

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Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 203-209 doi: 10.1007/s11705-012-1277-2

摘要： Antifungal activity of recombinant medicinal leech destabilase-lysozyme (rec.Dest-Lys) was investigated by using fungi: , and , including yeasts and . Its antibacterial activity was investigated on gram-negative bacteria . These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec.Dest-Lys disappeared after heat-treating at 90°C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative of rec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.

关键词： recombinant destabilase-lysozyme (rec.Dest-Lys)     antimicrobial activity     antifungal activity     medicinal leech     heated-up derivative of rec.Dest-Lys (T-rec.Dest-Lys)    

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Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization study for biodiesel production

Lim, Peck L. Kiew, Steven Lim, Siti S. Rosli, Chung Y. Wong, Pau L. Show, Keat T. Lee

《化学科学与工程前沿（英文）》 2021年 第15卷 第5期   页码 1257-1268 doi: 10.1007/s11705-020-2015-9

摘要： In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells ( ) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·h ozone concentration, 30 min ozonation time, 1 L·min of ozone flowrate at medium pH of 10 and temperature of 30 °C), the sedimentation efficiency of microalgae cells increased significantly from 12.56% to 68.62%. It was observed that the microalgae cells aggregated to form flocs after pre-treated with ozone due to the increment of surface charge from –20 to –6.59 mV. Besides, ozone had successfully disrupted the microalgae cells and resulted in efficient lipid extraction, which was 1.9 times higher than the control sample. The extracted microalgae lipid was mainly consisted of methyl palmitate (C16:0), methyl oleate (C18:1) and methyl linolenate (C18:3), making it suitable for biodiesel production. Finally, utilization of recycled culture media after ozonation pre-treatment showed robust growth of microalgae, in which the biomass yield was maintained in the range of 0.796 to 0.879 g·h for 5 cycles of cultivation.

关键词： microalgae     biodiesel     ozonation     pre-treatment     lipid    

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Reduced texaphyrin: A ratiometric optical sensor for heavy metals in aqueous solution

Root, Gregory Thiabaud, Jonathan L. Sessler

《化学科学与工程前沿（英文）》 2020年 第14卷 第1期   页码 19-27 doi: 10.1007/s11705-019-1888-y

摘要： We report here a water-soluble metal cation sensor system based on the as-prepared or reduced form of an expanded porphyrin, texaphyrin. Upon metal complexation, a change in the redox state of the ligand occurs that is accompanied by a color change from red to green. Although long employed for synthesis in organic media, we have now found that this complexation-driven redox behavior may be used to achieve the naked eye detectable colorimetric sensing of several number of less-common metal ions in aqueous media. Exposure to In(III), Hg(II), Cd(II), Mn(II), Bi(III), Co(II), and Pb(II) cations leads to a colorimetric response within 10 min. This process is selective for Hg(II) under conditions of competitive analysis. Furthermore, among the subset of response-producing cations, In(III) proved unique in giving rise to a ratiometric change in the ligand-based fluorescence features, including an overall increase in intensity. The cation selectivity observed in aqueous media stands in contrast to what is seen in organic solvents, where a wide range of texaphyrin metal complexes may be prepared. The formation of metal cation complexes under the present aqueous conditions was confirmed by reversed phase high-performance liquid chromatography, ultra-violet-visible absorption and fluorescence spectroscopies, and high-resolution mass spectrometry.

关键词： texaphyrin     fluorescent sensor     ion-sensing     indium     mercury    

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标题 作者 时间 类型 操作

Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

Quanlin DAI, Baifei HUANG, Zhongyi YANG, Jiangang YUAN, Junzhi YANG

期刊论文

深碳观测计划——对地球内部碳的十年探索

Schiffries, Andrea Johnson Mangum, Jennifer L. Mays, Michelle Hoon-Starr, Robert M. Hazen

期刊论文

In memory of Perry L. McCarty, seminal founder of Environmental Biotechnology

期刊论文

Comments on the special issue on forestry of

John L. INNES

期刊论文

Shear design of high strength concrete prestressed girders

Emad L. LABIB,Hemant B. DHONDE,Thomas T. C. HSU,Y. L. MO

期刊论文

Deeply missing my career idol and role model ― Professor Perry L. McCarty

期刊论文

Urban watershed management under rapid urbanization

Pengfei DU, Haifeng JIA, Shaw L. YU

期刊论文

地下工程的发展提升现代生活质量

Raymond L. Sterling

期刊论文

A Standard for the Commissioning Process

William L. Gillis,Elizabeth A. Cudney

期刊论文

The Decarbonization of Construction—How Can Alkali-Activated Materials Contribute?

John L. Provis,Susan A. Bernal,Zuhua Zhang,

期刊论文

Quality Function Deployment Implementation in Construction: A Systematic Literature Review

Cudney,William L. Gillis

期刊论文

Effects of iron oxide nanoparticles on phenotype and metabolite changes in hemp clones ( L.)

期刊论文

Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

期刊论文

Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization study for biodiesel production

Lim, Peck L. Kiew, Steven Lim, Siti S. Rosli, Chung Y. Wong, Pau L. Show, Keat T. Lee

期刊论文

Reduced texaphyrin: A ratiometric optical sensor for heavy metals in aqueous solution

Root, Gregory Thiabaud, Jonathan L. Sessler

期刊论文